Singular Knowledge Base
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Running the G4X
NGS Library Preparation
Sample Input
Library Prep Recommendations
Singular Adapters and Primers
Library Prep Partners
Size Selection and Cleanup
Library Quality Control
Running the G4X
Designing a Sequencing Run
Sample Sheet
Performing a Run
System Maintenance
System Troubleshooting
NGS Analysis on G4X
Data Output
PhiX Data
Demultiplexing and Filtering
G4X Reagents
Sequencing Reagents
Specifications and Capabilities
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Singular Knowledge Base
Running the G4X
NGS Library Preparation
Sample Input
Library Prep Recommendations
Singular Adapters and Primers
Library Prep Partners
Size Selection and Cleanup
Library Quality Control
Running the G4X
Designing a Sequencing Run
Sample Sheet
Performing a Run
System Maintenance
System Troubleshooting
NGS Analysis on G4X
Data Output
PhiX Data
Demultiplexing and Filtering
G4X Reagents
Sequencing Reagents
Specifications and Capabilities
Running the G4X
This section will guide you though how to properly plan for a G4X run, load the instrument and start sequencing.
Designing a Sequencing Run
What Read Length Do I Choose?
What Should I Consider When Pooling Libraries?
What Depth of Coverage Do I Need?
What Do I Consider When Designing a Sequencing Run?
What Read Type Do I Choose?
Why Would I Multiplex?
Can I load different samples with the same index on a single flow cell if the samples are split across different lanes?
Is the usage of read length flexible within a run and are asymmetrical reads supported?
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Sample Sheet
What Does the Sample Sheet Contain?
How Do I Create a Sample Sheet?
What Do I Do if I Cannot Select the Sample Sheet?
How Do I Specify Custom Demultiplexing in the Sample Sheet?
What Are the Differences Between G4 and Third-party Sample Sheets for Index Sequencing
What is the format for the G4 Sample Sheet?
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Performing a Run
What Do I Do if the Flow Cell Does Not Engage?
What Do The Flow Cell Compartment Lights Mean?
What Do I Do if the External Waste Reservoir Is Not Empty?
What Do I Do if the Product Identifier Is Not Recognized?
How Do I Load Custom Sequencing Primers?
What Do I Do if the Internal Waste Reservoir Is Not Empty?
What Do I Do if the Flow Rate Check Failed?
Why Is Titration of the Loading Concentration Important?
How Do I Prepare Sequencing Reagents?
Why Is the Loading Concentration Important?
How much library should I load per lane onto the G4 Sequencing Platform?
How much library should be loaded for G4 runs?
How to quantify and QC Singular libraries?
What is the ideal sample loading concentration for the G4 Sequencing Platform?
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System Maintenance
How Do I Perform Storage Maintenance?
How Do I Perform a Maintenance Wash or Rinse?
When Do I Perform Software Updates?
System Troubleshooting
How Do I Contact Customer Care?
How Do I Shut Down the Instrument?
How Do I Restart the Instrument and Primary Computer?
How Do I Start Up the Instrument?
