Singular Knowledge Base
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NGS Analysis on G4X
NGS Library Preparation
Sample Input
Library Prep Recommendations
Singular Adapters and Primers
Library Prep Partners
Size Selection and Cleanup
Library Quality Control
Running the G4X
Designing a Sequencing Run
Sample Sheet
Performing a Run
System Maintenance
System Troubleshooting
NGS Analysis on G4X
Data Output
PhiX Data
Demultiplexing and Filtering
G4X Reagents
Sequencing Reagents
Specifications and Capabilities
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Singular Knowledge Base
NGS Analysis on G4X
NGS Library Preparation
Sample Input
Library Prep Recommendations
Singular Adapters and Primers
Library Prep Partners
Size Selection and Cleanup
Library Quality Control
Running the G4X
Designing a Sequencing Run
Sample Sheet
Performing a Run
System Maintenance
System Troubleshooting
NGS Analysis on G4X
Data Output
PhiX Data
Demultiplexing and Filtering
G4X Reagents
Sequencing Reagents
Specifications and Capabilities
NGS Analysis on G4X
This section will explore data output of the G4 and how to work with downstream bioinformatics pipelines
Data Output
What Are Unfiltered FASTQ Files?
What Are Demultiplexed FASTQ Files?
Where Are the Run Logs?
What Are the Results after Sequencing?
What Does the Run Folder Contain?
What Are FASTQ Files?
How many FASTQ files are there for a Max Read library?
Where can I find my raw or demultiplexed/filtered FASTQ files? What are the outputs generated by G4?
What data format does the G4 Sequencing Platform output?
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PhiX Data
How are PhiX reads processed by G4?
PhiX Statistics: How are they generated, and where are they located?
Demultiplexing and Filtering
How does the Singular Demultiplex software prevent barcode collisions?
How to pass the demultiplexing options through the sample sheet?
How is the read structure defined for demultiplexing?
What are the filtering and masking options for G4 demultiplexing?
What parameters in the Singular Demultiplex software control the stringency of read assignment?
How does folder autodetection work for the Singular Demultiplex software?
How Does Filtering Work?
How Do I Demultiplex Off-instrument?
What Are Quality Scores?
Where does the demultiplexing take place?
How do I re-demultiplex my run off-instrument?
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