What Depth of Coverage Do I Need?

Coverage and read requirements depend on several parameters.

In this guide, we define sequencing coverage as the number of unique reads that include a given nucleotide in the target region or genome of interest. Coverage and read requirements can depend on several of the following parameters:

• Application type

• Genome size, or in the case of target enrichment, region of interest/capture design size

• Genome complexity

• Read length

• Sequencing error rate

• Bioinformatics pipeline

Note

If you need more guidance regarding depth of coverage, contact Customer Care for consultation.

For more information, see the G4 Best Practices and Quality Control Guide, reach out to your Field Application Scientist, or contact Customer Care.

October 2023