Singular Knowledge Base
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NGS Library Preparation
NGS Library Preparation
Sample Input
Library Prep Recommendations
Singular Adapters and Primers
Library Prep Partners
Size Selection and Cleanup
Library Quality Control
Running the G4X
Designing a Sequencing Run
Sample Sheet
Performing a Run
System Maintenance
System Troubleshooting
NGS Analysis on G4X
Data Output
PhiX Data
Demultiplexing and Filtering
G4X Reagents
Sequencing Reagents
Specifications and Capabilities
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Singular Knowledge Base
NGS Library Preparation
NGS Library Preparation
Sample Input
Library Prep Recommendations
Singular Adapters and Primers
Library Prep Partners
Size Selection and Cleanup
Library Quality Control
Running the G4X
Designing a Sequencing Run
Sample Sheet
Performing a Run
System Maintenance
System Troubleshooting
NGS Analysis on G4X
Data Output
PhiX Data
Demultiplexing and Filtering
G4X Reagents
Sequencing Reagents
Specifications and Capabilities
NGS Library Preparation
This section will explore how to prepare NGS libraries for sequencing on the G4X and best practices in working with partners.
Sample Input
What Sample Quality Do I Need for Library Prep?
What Types of Samples Can I Use for Library Prep?
Library Prep Recommendations
How does Max Read sequencing work?
What are the Max Read PhiX Controls?
What Is the Singular Indexing Strategy?
What Fragmentation Method Can I Use for Library Prep?
What Are the Amplification Conditions for Library Prep?
What Is the Polymerase Fidelity Needed for Library Prep?
What Is the Insert Size Singular Supports?
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Singular Adapters and Primers
What are the Max Read Primer Sets?
How Do I Perform Singular Genomics Adapter Ligation?
Does the G4 Sequencing Platform support indexed libraries?
What are the sequences of Singular adapters?
Singular Genomics-Specific Blocking Oligos
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Library Prep Partners
How do I demultiplex when NEBNext Multiplex Oligos (Single Index) are used for library preparation?
NEBNext Ultra II FS DNA Library Prep Kit
Quantabio sparQ DNA Library Prep Kit
Phase Genomics Proximo Hi-C Kit
Fluent PIPseq 3' RNA kit
Agilent SureSelect Hybrid Captured Libraries
Paragon CleanPlex NGS Panel
Quantabio sparQ RNA-Seq HMR Kit
QIAseq miRNA Library Kit
Takara SMART-Seq V4 mRNA Library Prep
What 10x Genomics products are compatible with Max Read Sequencing?
Bio-Rad SEQuoia Stranded RNA Library Prep Kit
Watchmaker Library Prep
Parse Biosciences Evercode Whole Transcriptome (WT) Kit
10x Genomics Library Prep
How Do I Adapt Libraries for the G4™ While Retaining Original Indices?
How Do I Adapt Libraries for the G4™—Insert Only?
Claret SRSLY NGS Library Prep Kit
Tecan MagicPrep NGS System
NEBNext Ultra II Directional RNA Library Prep Kit
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Size Selection and Cleanup
What Is the Optimal Ratio When Using SPRI Beads?
How Do I Perform Size Selection?
How Do I Work with SPRI beads?
What Stringency of Fragment Removal Is Needed When Using SPRI Beads?
Library Quality Control
What Causes Bubble Products?
How Do I Perform Quality Control?
What Causes Presence of Adapter Dimers?
PCR-Free Library Prep Quality Control
Amplified Library Prep Quality Control
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