What Are the Amplification Conditions for Library Prep?

Choose amplification conditions according to the library prep manufacturer's instructions.

In general, limit the number of PCR cycles to reduce the potential for additional bias and error associated with the PCR process. However, ensure that you have sufficient library for sequencing, as well as for any quality control (QC) processes. Quality control processes could use BioAnalyzer, Fragment Analyzer, Qubit, qPCR, or other processes.

Note for PCR-free library methods, the final product may be at such a low yield that you are unable to perform all desired quality control. qPCR quantification is recommended for PCR-free libraries.

For more information, see the G4 Best Practices and Quality Control Guide, reach out to your Field Application Scientist, or contact Customer Care.

October 2023