What Is the Optimal Ratio When Using SPRI Beads?

Several factors can influence the optimal ratio, including sample concentration, sample diluent, sample volume, and fragment size distribution.

To determine the optimal size selection conditions, consider performing a titration series using different ratios (for example, 1.0×, 0.9×, 1×, 0.8×) for each new type of library.

The library size distribution should be checked using the Bioanalyzer DNA HS Chip or equivalent. Ensure the analysis shows the size on the x-axis and consider using tools to visualize and quantify the areas under the curve if any bimodality is observed.

For more information, see the G4 Best Practices and Quality Control Guide, reach out to your Field Application Scientist, or contact Customer Care.

October 2023