Q: Are Singular-specific blockers available for purchase?
A: Yes, Singular Genomics-specific blockers from IDT are available.
To request hybrid capture blocking oligos from IDT specific for libraries compatible with the G4 Sequencing Platform, please email ngsdesign@idtdna.com and include the following sequences:
ACAAAGGCAGCCACGCACTCCTTCCCTGTXXXXXXXXXXXXXACACTCTTTCCCTACACGACGCTCTTCCGATCT/3block
CTCCAGCGAGATGACCCTCACCAACCACTXXXXXXXXXXXXXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT/3block
NOTE: Blocking oligos are made and shipped as 25 reaction aliquots (IDT suggests 1 μL = 1 reaction). If you need more, request multiples of 25. Oligos arrive lyophilized, and IDT recommends resuspension in IDTE pH. 8.0 (10 mM Tris, pH 8.0, 0.1 mM EDTA).
Version: 29 Sep 2023