The size selection cut-off is determined by the volume-to-volume ratio of bead suspension to DNA solution. A lower ratio results in a higher size cut-off, that means, a more stringent removal of the smaller size molecules.
- Singular Knowledge Base
- Library Preparation
- Size Selection and Cleanup
How Do I Perform Size Selection?
Small molecular species such as free adapters and adapter dimers are removed using SPRI beads (for example, AMPure XP or equivalent).
For more information, see the G4 Best Practices and Quality Control Guide, reach out to your Field Application Scientist, or contact Customer Care.
October 2023