Q: How do I adapt the Quantabio sparQ RNA-Seq HMR Kit to sequence on the G4 Sequencing Platform?
A: The Quantabio sparQ RNA-Seq HMR Kit can be adapted for the G4 Sequencing Platform using the protocol listed below.
LIBRARY PREPARATION PROTOCOL
For a protocol for Quantabio sparQ RNA-Seq HMR Library Prep sequencing experiments on the G4 Sequencing Platform, see:
Quantabio sparQ RNA-Seq HMR Kit for Singular Genomics G4 Sequencing
DEMONSTRATED G4 SEQUENCING EXPERIMENTS
For demonstrated Quantabio sparQ RNA-Seq HMR Library Prep sequencing experiments on the G4 Sequencing Platform, see the App Note:
ORDERING INFORMATION
Singular Genomics (SG) indexed and non-indexed PCR primers and adapters can be purchased from Singular Genomics:
Part #
Product
SG Universal Library Prep Adapter + UDI Primers (1-96)
SG Universal Library Prep Adapter + UDI Primers Set A (1-24)
SG Universal Library Prep Adapter + UDI Primers Set B (25-48)
SG Universal Library Prep Adapter (96 Reactions)
SG Universal Library Prep Adapter (24 Reactions)
SG Non-Indexed Library Prep Primers (24 Reactions)
Quantabio sparQ RNA-Seq HMR Kit can be purchased from Quantabio:
Part #
Product
Quantabio sparQ RNA-Seq HMR Kit
Version: 18 Dec 2023