Demultiplexing and Filtering
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      3. Demultiplexing and Filtering

      Where does the demultiplexing take place?

      This article describes the process for demultiplexing data on the G4 Sequencing Platform

      Demultiplexing will be performed on the instrument by default using the sample data sheet you entered during run setup. If you don’t provide a sample data sheet, the files will just be filtered and not demultiplexed.

      Both the raw FASTQ files as well the demultiplexed FASTQ files will be transferred to the output location you have defined in the instrument settings.

      Singular Genomics also offers the option to demultiplex FASTQ files off-instrument for more advanced demultiplexing settings. You can use the Singular Demultiplexer or the Singular Demultiplex Workflow to re-demultiplex off Instrument.

      See also the topic Fixing Improper Demultiplexing in the G4 Sequencing Platform User Guide.

       

      Note: this article is part of our Guide to Working With G4 Sequencing Data Technical Bulletin.