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Running NGS on the G4X
This section will guide you though how to properly plan for a G4X NGS run, load the instrument and start sequencing.
Designing a Sequencing Run
- Can I load different samples with the same index on a single flow cell if the samples are split across different lanes?
- What Read Length Do I Choose?
- What Should I Consider When Pooling Libraries?
- Is the usage of read length flexible within a run and are asymmetrical reads supported?
- What Depth of Coverage Do I Need?
- What Do I Consider When Designing a Sequencing Run?
- What Read Type Do I Choose?
- Why Would I Multiplex?
Sample Sheet
- What Does the Sample Sheet Contain?
- What is the format for the G4 Sample Sheet?
- How Do I Create a Sample Sheet?
- What Do I Do if I Cannot Select the Sample Sheet?
- How Do I Specify Custom Demultiplexing in the Sample Sheet?
- What Are the Differences Between G4 and Third-party Sample Sheets for Index Sequencing
Performing a Run
- What Do I Do if the Flow Cell Does Not Engage?
- How much library should I load per lane onto the G4 Sequencing Platform?
- What Do The Flow Cell Compartment Lights Mean?
- How much library should be loaded for G4 runs?
- How to quantify and QC Singular libraries?
- What Do I Do if the External Waste Reservoir Is Not Empty?
- What Do I Do if the Product Identifier Is Not Recognized?
- What is the ideal sample loading concentration for the G4 Sequencing Platform?
- How Do I Load Custom Sequencing Primers?
- What Do I Do if the Internal Waste Reservoir Is Not Empty?
- What Do I Do if the Flow Rate Check Failed?
- Why Is Titration of the Loading Concentration Important?
- How Do I Prepare Sequencing Reagents?
- Why Is the Loading Concentration Important?