In this guide, we define sequencing coverage as the number of unique reads that include a given nucleotide in the target region or genome of interest. Coverage and read requirements can depend on several of the following parameters:

• Application type

• Genome size, or in the case of target enrichment, region of interest/capture design size

• Genome complexity

• Read length

• Sequencing error rate

• Bioinformatics pipeline

Note

If you need more guidance regarding depth of coverage, contact Customer Care for consultation.