Why Is Titration of the Loading Concentration Important?
For larger experiments, or for previously uncharacterized library types, you consider performing a titration run to determine the optimal loading concentration for a library of interest.
A typical titration series consists of 10 pM, 15 pM, 20 pM, 25 pM, and 30 pM loading concentrations.
For more information, see the G4 Best Practices and Quality Control Guide, reach out to your Field Application Scientist, or contact Customer Care.
October 2023