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NGS Analysis on G4X
This section will explore data output of the G4 and how to work with downstream bioinformatics pipelines
Data Output
- Where can I find my raw or demultiplexed/filtered FASTQ files? What are the outputs generated by G4?
- What Are Unfiltered FASTQ Files?
- What data format does the G4 Sequencing Platform output?
- What Are Demultiplexed FASTQ Files?
- Where Are the Run Logs?
- What Are the Results after Sequencing?
- What Does the Run Folder Contain?
- What Are FASTQ Files?
- How many FASTQ files are there for a Max Read library?
PhiX Data
Demultiplexing and Filtering
- How does the Singular Demultiplex software prevent barcode collisions?
- Where does the demultiplexing take place?
- How to pass the demultiplexing options through the sample sheet?
- How do I re-demultiplex my run off-instrument?
- How is the read structure defined for demultiplexing?
- What are the filtering and masking options for G4 demultiplexing?
- What parameters in the Singular Demultiplex software control the stringency of read assignment?
- How does folder autodetection work for the Singular Demultiplex software?
- How Does Filtering Work?
- How Do I Demultiplex Off-instrument?
- What Are Quality Scores?