To determine the optimal size selection conditions, consider performing a titration series using different ratios (for example, 1.0×, 0.9×, 1×, 0.8×) for each new type of library.

The library size distribution should be checked using the Bioanalyzer DNA HS Chip or equivalent. Ensure the analysis shows the size on the x-axis and consider using tools to visualize and quantify the areas under the curve if any bimodality is observed.